肝素对重氮法总胆红素测定结果的影响

Influence of Heparin on Detection Total Bilirubin with Heavy Nitrogen

目的:探讨肝素对重氮法总胆红素测定结果影响。方法:用双波长双试剂法和双波长单一剂法测定各添加肝素0,6250,12500 U/L,同一质控物各20次。结果:肝素对重氮法总胆红素测定结果存在干扰,使结果呈假性增高。结论:肝素与总肝红素试剂中的十二烷基二甲基溴化铵反应,形成不溶于水的白色混悬物,造成假性增高。双波长双试剂法优于双波长单一试剂法。

Objective To observe the influence of heparin on detecting total bilirubin with heavy nitrogen. Methods The control samples contained a succession of concentration of heparin were detected 20 times with double wavelength-double reagent and double wavelength-single reagent respectively. Results Heparin had a influence on detecting total bilirbin with heavy nitrogen. Conclusion Heparin makes the result increase due to it reacting with dodecydimethylammorium bromides. Double wavelength-double reagent is better than double wavelength-single reagent.