摘要
To study the role of interleukin(IL)-10 in acute-graft-versus-host disease (aGVHD) and graft rejection Methods Serum concentrations of IL-10 in 28 patients who underwent allogeneic hematopoietic stem cell transplantation (allo-HSCT) were measured by enzymed-linked immunosorbent assay (ELISA) IL-10 gene expression in peripheral mononuclear cells was measured by reverse-transcriptase polymerase chain reaction (RT-PCR) after transplantation Results Seven patients developed grade Ⅰ GVHD, 7 patients developed grade Ⅱ-Ⅳ GVHD, 4 patients had graft rejection Before transplantation, the concentrations of IL-10 were higher in patients who later did not developed aGVHD After transplantation, IL-10 levels increased in patients without aGVHD, but decreased in patients with aGVHD or graft rejection And IL-10mRNA was more frequent in patients without aGVHD compared to those with aGVHD Conclusions IL-10 plays a negative role in the development of aGVHD and graft rejection
To study the role of interleukin(IL)-10 in acute-graft-versus-host disease (aGVHD) and graft rejection Methods Serum concentrations of IL-10 in 28 patients who underwent allogeneic hematopoietic stem cell transplantation (allo-HSCT) were measured by enzymed-linked immunosorbent assay (ELISA) IL-10 gene expression in peripheral mononuclear cells was measured by reverse-transcriptase polymerase chain reaction (RT-PCR) after transplantation Results Seven patients developed grade Ⅰ GVHD, 7 patients developed grade Ⅱ-Ⅳ GVHD, 4 patients had graft rejection Before transplantation, the concentrations of IL-10 were higher in patients who later did not developed aGVHD After transplantation, IL-10 levels increased in patients without aGVHD, but decreased in patients with aGVHD or graft rejection And IL-10mRNA was more frequent in patients without aGVHD compared to those with aGVHD Conclusions IL-10 plays a negative role in the development of aGVHD and graft rejection
基金
ThisstudywassupportedbyagrantfromNationalNaturalSciencesFoundationofChina (No 39870 71 0 )
