逼尿肌不稳定缝隙连接蛋白基因及其蛋白的表达意义

Expression of gap junction gene connexin and its protein in detrusor instability

目的 研究膀胱逼尿肌缝隙连接蛋白 (Cx)基因及其蛋白的表达与逼尿肌不稳定(DI)发生的关系。方法 应用逆转录 聚合酶链反应 (RT PCR)技术及Western blot方法 ,从转录及翻译水平检测细胞间缝隙连接蛋白基因及其蛋白在DI中的表达变化。结果 DI组Cx43、Cx40mRNA表达水平高于正常对照组 (P <0 .0 1) ,而Cx45mRNA表达差异无显著性 (P >0 .0 5 ) ;Cx43蛋白表达量DI组也明显高于正常对照组 (P <0 .0 1)。结论 连接蛋白基因及其蛋白在DI中表达增高 。

Objective To study the relationship between the expression of gap junction gene connexin and its protein in bladder detrusor and pathogenesis of detrusor instability (DI).Methods RT PCR and Western blot techniques,in transcript and protein levels,were employed to detect the expression of connexin and its protein in DI.Results The levels of Cx43,Cx40 mRNA expression in DI groups were higher than in control groups ( P <0.01).There was no significant difference in Cx45 mRNA between two groups ( P >0.05).Positive rate of Cx43 protein in DI group was significantly higher than in control group ( P <0.01).Conclusion There is an increase in connexin transcript and protein levels in detrusor instability,suggesting that the increase of intercellular excitatory communication is associated with DI.