P^(27)蛋白在中耳乳突胆脂瘤上皮中的表达及意义

P27 Protein expression and its significance in human middle ear cholesteatoma

目的 探讨P^(27)蛋白在中耳继发性胆脂瘤上皮中的表达,分析其在胆脂瘤上皮增生调节中的可能作用。方法 运用免疫组化En Vision染色法和计算机图像分析法,检测32例胆脂瘤上皮和9例胆脂瘤患者外耳道皮肤中P^(27)蛋白的表达情况。结果 P^(27)表达在胆脂瘤上皮及外耳道皮肤的棘层及颗粒层的细胞核,两种组织P^(27)阳性表达的平均灰度值(±s,下同)分别为(15.6983±6.991)和(153.138 6±4.702 2),两者差异有显著意义(P<0.01)。结论 P^(27)胆脂瘤上皮的低表达有可能是胆脂瘤上皮细胞高度增殖的原因之一。

Objective To evaluate the role of p27 protein in acquired middle ear cholesteatoma. Methods The specimens from the acquired middle ear cholesteatoma tissue of 32 cases and the ear canal epithelium of 9 cases were examined by immuohistochemical (EnVision method) and computer image analysis. Results P27 positive cells were expressed in the prickle cell layer and stratum granulosum cell layer of cholesteatoma epithelium and ear canal epithelium. The level of P27 protein were 159. 698 3± 6. 991 and 153. 138 6±4. 702 2 respectively. The results had very significant difference (P<0. 01). Conclusion Less expression of P27 in cholesteatoma may be a cause of the epithelium hyperplasia.