异丙酚早期给药对内毒素休克大鼠的保护效应

The protective effects of early treatment with propofol on endotoxin-induced shock in rats

目的 观察不同时点给予异丙酚对内毒素休克大鼠的作用及机制。方法 静脉给予脂多糖(LPS)8 mg·kg^(-1) 复制内毒素休克模型,雄性 Wistar大鼠76只随机分5组:对照组(A组);LPS组(B组);C、D、E组为异丙酚+LPS组,分别于LPS注入前1h、LPS注入即刻、LPS注入后1h,均静注异丙酚5 mg·kg^(-1),继以10 mg·kg^(-1)·h^(-1)持续泵注。观察动物 MAP、PaO_2,pH,血清肿瘤坏死因子(TNF-α)、一氧化氮(NO)浓度,肺组织TNF-α及丙二醛(MDA)含量、髓过氧化物酶(MPO)活性的变化。并比较各组存活率。结果 与B组比较,C、D组可不同程度地逆转MAP、PaO_2 、pH的下降,抑制血清TNF-α、NO水平及肺组织TNF-α、MDA、MPO水平的升高,动物存活率提高(P<0.01或0.05)。E组上述指标改善较C组、D组弱。结论 异丙酚早期给药对内毒素休克起保护效应。

Objective To investigate the effects of propofol administered at different intervals on acute lung injury induced by endotoxin shock and the mechanism in rats.Methods Seventy-six healthy adult male Wistar rats, weighing 250-290 g were anesthetized with 30 % pentobarbital sodium 30 mg. kg-1 ip. Carotid artery and jugular vein were cannulated for BP monitoring and administration of drugs and fluid. The animals were randomly divided into 5 groups : group A received only normal saline (NS) ( n = 8) ; group B received LPS 8 mg' kg and NS iv ( n =17); group C-E received a bolus of propofol 5 mg .kg-1 iv, 1 h before (group C) or together with (group D) or 1 h after intravenous LPS (group E) followed by propofol infusion at 10 mg . kg-1 . h-1 ( n = 17 in each group). The animals were observed for 5 h after LPS. Blood samples were taken from femoral artery before (baseline) and 1, 3 , 5 h after LPS for determination of PaO2 ,pH, and serum TNF-a and NO levels. The survival rates of the animals in the 5 groups during the 5 h after LPS were compared. The animals were sacrificed and the lungs were removed at the end of 5 hour observation for determination of lung TNF-a and MDA content and myeloperoxidase (MPO) activity and microscopic examination. Results Propofol given 1h before (group C) and simultaneously with LPS (group D) significantly attenuated the decrease in MAP, pH and PaO2 and increase in serum contents of TNF-a and NO and lung TNF-a, MDA contents and MPO activity induced by LPS. The 5 hour survival rate was also significantly higher in group C and D than that in group B. Conclusion Early propofol administration is beneficial during endotoxin-induced shock.