PKCβ1和PKCβ2在早期胃癌中的表达

Expression of PKC β1 and PKC β2 in early gastric carcinoma

目的:观察PKC β1和PKC β2 在早期胃癌癌组织及其癌周不典型增生和肠上皮化生中的表达。方法:对42例早期胃癌胃全切标本的癌组织、癌旁不典型增生、肠上皮化生及正常胃黏膜进行PKC β1和PKC β2免疫组织化学染色。结果:正常胃黏膜上皮颈部散在PKC β1阳性细胞,黏膜表面上皮细胞及幽门腺PKC β1和 PKC β2阴性;胃底腺壁细胞和主细胞PKC β1和 PKC β2呈强阳性。胃黏膜内淋巴滤泡中心区PKC β1强阳性,PKC β2阴性;相反,淋巴滤泡帽带区PKC β1阴性,而PKC β2强阳性。胃癌、癌旁不典型增生、肠上皮化生PKC β1的表达明显增强,而PKC β2的表达无明显增强。肠型胃癌PKC β1的表达明显高于弥漫型。PKC β1的表达与早期胃癌的浸润深度无关。结论:PKC β1在胃黏膜腺上皮颈部和淋巴滤泡中心的强阳性表达提示PKC β1可能与细胞的增生活性有关;胃癌及癌前病变中PKC β1的表达增强显示PKC β1参与了胃癌的癌变过程,并可能在癌变早期发生改变。

AIM: To study the expression of PKC β1 and PKC β2 in carcinoma tissue, dysplasia and intestinal metaplasia of early gastric carcinoma. METHODS: Forty two cases of early gastric carcinoma were randomly selected from the file of PUMH during 1998-2001. The expression of PKC β1 and PKC β2 was investigated by immunohistochemistry method. The staining pattern of PKC β1 and PKC β2 was compared among carcinoma,dysplasia, intestinal metaplasia,and normal gastric tissue. RESULTS: In normal gastric mucosa, PKC β1 dnd PKC β2 were strongly positive in fundic glandular epithelium, but negative in pyloric and entire surface of mucine-secretion epithelial cells. PKC β1 showed positive staining not only in the glandular cells at the neck region and but also in the follicular center cells of lymphoid tissue. However, PKC β2 was negative in those areas. Most importantly, PKC β1 demonstrated much stronger staining in carcinoma, dysplasia and intestinal metaplasia, especially in intestinal carcinoma, compared to normal gastric mucosa. There was no sig- niffcant difference of PKC β1 positivity between intramucosa carcinoma and submucosa carcinoma. CONCLUSION: The expression of PKC β1 may be related to the proliferation of gastric epithelium. Our findings strongly indicate that elevated expression of PKC β1 may be an early event in the pathway of gastric carcinogenesis.