胃黏膜癌变过程中PTEN基因编码产物的表达及意义

Expression of PTEN encoding product in malignant lesions of gastric mucosa and its significance

目的:观察抑癌基因PTEN编码产物在胃黏膜癌变过程中的表达,探讨PTEN表达与胃癌发生的关系。方法:选取胃镜下正常胃黏膜、慢性浅表性胃炎、萎缩性胃炎无肠化、萎缩性胃炎伴肠化、中度不典型增生、重度不典型增生标本各60例,选取手术后早期胃癌、进展期胃癌标本各60例,应用S-P免疫组化方法检测各种胃黏膜病变中PTEN编码产物表达,比较其表达与胃癌发生的关系。结果:PTEN编码产物在正常胃黏膜、慢性浅表性胃炎、无肠化萎缩性胃炎、伴肠化萎缩性胃炎、中度异型增生、重度异型增生、早期胃癌和进展期胃癌中的阳性表达率分别为 100％,98.3％,91.6％,78.3％,75％,63.3％,61.7％,43.3％。在检测的 120例胃癌中,肠型胃癌76例,PTEN表达率为60.5％,弥漫型胃癌44例,PTEN表达率为 38.6％。结论:PTEN基因编码蛋白在胃癌发生过程中进行性下调,PTEN蛋白表达可作为判定胃癌生物学行为的客观指标。

AIM: To observe the expression of PTEN protein in gastric cancer and precancerous lesions, and to investigate the relationship between PTEN expression and the pathogenesis of gastric cancer. METHODS: Normal gastric mucosa,chronic superficial gastritis, atrophic gastritis without intestinal metaplasia, atrophic gastritis with intestinal metaplasia, moderate and severe dysplasia, early and advanced gastric cancer, 60 cases each group, were selected for PTEN protein expression by SP immunohistochemistry. RESULTS: The expression of PTEN encoding product in normal gastric mucosa was 100 %. For chronic superficial gastritis, atrophic gastritis without intestinal metaplasia, atrophic gastritis with intestinal metaplasia, moderate dysplasia, severe dysplasia, the PTEN protein expression rate was 98.3 %, 91.6 %, 78.3 %, 75 %, 63.3%,respectively. The expression of PTEN protein in early stage and advanced gastric cancer was 61.7 % and 43.3 % respectively. Among the 120 cases of gastric cancer, 76 cases were intestinal type gastric cancer, the PTEN protein expression was 60.5%, 44 cases were diffuse gastric cancer, the PTEN protein expression was 38.6 %. CONCLUSION: The expression of PTEN protein is downregulated in the process of gastric cancer, PTEN protein can be used as a maker to evaluate the biological behaviours of gastric cancer.