胃癌中医证型相关基因的表达谱

Gastric cancer related gene expression spectrum in disease pattern of traditional Chinese medicine

目的:寻找不同胃癌证型组织中的相关基因表达谱差异表达,对研究胃癌证型实质进行探讨、胃癌的中医证的基因诊断及指导临床用药有重大的意义。方法:我们运用含有4 096条人类全长基因的cDNA表达芯片技术,对胃癌中医证型邪热内蕴证和气滞血瘀证共4例,胃癌组织及胎胃正常组织基因表达进行了分析。结果:两证型都有免疫相关基因的下降,也有癌基因的上调,但热毒内蕴亚型基因表达提示了正气未虚,尚有防御能力,如nm23基因增高,P^(18)基因表达增高;而气虚夹瘀亚型中则无此改变。结论:胃癌患者的基因表达既有相同表达改变,也有不同中医证型的基因谱表达改变也不相同,基因表达谱可为中医的证型提供科学依据。

AIM: To find difference in gene expression pedigree of gastric carcinoma tissue and different syndrome, and its significance in the exploration of the syndrome typ and diagnosisof gastric carcinoma, with gene diagnosis as well as the use of drugs in its treatment. METHODS: The gene expression in two TCM syndrome types of gastric carcinoma due to internal stagnancy of pathogenic heat and stagnancy of qi and blood stasis, respectively, including 4 human gastric carcinoma tissues and normal embryo stomach tissue, was analyzed by using 4096 pieces of human full-length genic CDNA expression CMOS chip technology. RESULTS: The gene expression in the two TCM syndrome types of gastric carcinoma was decreased in their immune associated genes, but increased in cancer-associated genes. Gene expression in the interior stagnancy of toxic heat showed that the body resistance was not weakened, suggesting that there was no deficiency of qi and stagnancy of blood stasis CONCLUSION: The change in gene expression of gastric cancer is identical, but the gene expression spectrum is different in different types of TCM syndromes.