直肠癌组织CD44v6,DNA含量的联合检测及临床意义

Simultaneous detection of CD44v6 expression and DNA content in rectal cancer and its clinical significance

目的:探讨直肠癌CD44v6表达情况和DNA含量及其临床意义.方法:应用流式细胞术同时检测40例直肠癌及10例正常组织中CD44v6 表达量和DNA含量.结果:直肠癌中CD44v6表达量和DNA含量均显著高于正常组织(70％vs 0％,1.11± 0.6 vs 0.9±0.65,P<0.01),肿瘤组织中CD44v6阳性表达与直肠癌浸润深度(0％vs50％vs 80％vs 100％)、淋巴结转移(93.3％vs 56.0％)及Dukes分期(56.0％vs 93.3％)有关(P<0.05);DNA异倍体与淋巴结转移(100％vs 68％)及 Dukes分期(68％vs 100％)有关(P<0.05).CD44v6表达与肿瘤细胞DNA倍体无关(85.7％vs68.7％,P>0.05).结论:CD44v6表达异常及DNA倍体异常在直肠癌进展和浸润转移中起作用,联合检测CD44v6和DNA倍体可作为预测直肠癌进展程度、淋巴结转移有用的指标.

AIM: To explore CD44v6 expression and DNA content and their significance in the progress of rectal cancer. METHODS: In 40 cases of rectal cancer and 10 cases of normal intestinal tissues, CD44v6 expression and DNA content were detected simultaneously by flow cytometry. RESULTS: The CD44v6 expression and DNA content in rectal cancer were significantly higher than those in normal tissue (70 % vs 0 %, 1.11±0.6 vs 0.9±0.65. P<0.01). The positive expression of CD44v6 was closely related to the depth of invasion (0 % vs 50 % vs 80 % vs 100 %, P<0.05) lymphnode metastasis (93.3 % vs 56.0 %, P<0.05) and Dukes stage (56.0 % vs 93.3 %, P<0.05). The DNA aneuploidy in cancer was also related to lymph node metastasis (100 % vs 68 %, P<0.05) and Duke's stage (68 % vs 100 %, P<0.05). The expression of CD44v6 did not show any correlation with DNA ploidy of tumor cells (85.7 % vs 68.7 %, P>0.05). CONCLUSION: The abnormal expression of CD44v6 and DNA aneuploidy plays an important role in the development, invasion and metastasis of rectal cancer. The detection of CD44v6 and DNA content simultaneously may serve as a useful indicator to predict progress of tumor and lymph node metastasis.