卫氏并殖吸虫成虫重组抗原的表达与鉴定

THE EXPRESSION AND IDENTIFICATION OF PARAGONIMIASIS WESTERMANI ADULT RECOMBINANT ANTIGEN

目的 表达已转化入大肠杆菌的卫氏并殖吸虫成虫抗原 (Pw Ag)基因 ,并评价其应用于免疫学诊断的价值。 方法 用 IPTG诱导表达已亚克隆入表达载体 p RESETB的卫氏并殖吸虫成虫抗原基因 ,以 SDS- PAGE和 Western blot分析鉴定表达产物。 结果 SDS- PAGE电泳可见 32 ku处有 1条明显的蛋白质带 ,该带只被卫氏并殖吸虫成虫免疫兔、感染犬和病人血清识别。 结论 成功构建重组克隆 Pw Ag,其表达产物具有卫氏并殖吸虫成虫期特异性 ,推测可用于并殖吸虫病的免疫学诊断。

Objective To express and identify the gene of Paragonimiasis westermani adult antigen(PwAg) which have converted into Escherichia coli (BL21) and evaluate its diagnostic value. Methods To induce the recombinant Pw/pRESETB/ E. coli BL21 expressed by IPTG and analyze the expressed protein with SDS PAGE and Western blot. Results A specific protein belt was shown at about 32 ku and the protein belt can be recoganised only by the sera of the rabbits immunized with PwAg, the dogs infected with P. westermani and the patients with paragonimiasis. Conclusion The Pw/pRESETB/ E. coli BL21 encoding specific PwAg has been established successfully.