4Staub A M. Removal of proteins from polysaccharides [ J]. Methods Incarbohydr Chem, 1965,23:55.
5Bayne C J. Pronephric leucocytes of Cyprinus carpio: Isolation, separation and characterization [ J]. Veterinary Immunology and Immunopathology, 1986,12 : 141 - 151.
6Shim K J,Jung K H, Chung M K,et al. Development of an in vitro environmental monitoring system by using immune cells [J ]. J Health Sci,2002,48(2) :130 -133.
7Secombes C J. Isolation of salmonid macrophages and analysis of their killing activity [ C ] // Stolen J S, Fletcher T C,Anderson D P,et al. Techniques in fish immunology Ⅲ. NJ : SOS Publications, 1990 : 138 - 154.
8Green L C,Wagner D A, Glogowski J,et al. Analysis of nitrate, nitrite, and [ 15N] nitrate in biological fluids [J]. AnalBiochem,1982,126:131-138.
9Mosmann T. Rapid colorimetic assay for celluar grow than survival:Application to proliferation and cytotoxicity assay [ J ]. J Immunol Methods, 1983,65:55 - 63.
10Yin G J, Ardo L, Thompson K D,et al. Chinese herbs (Astragalus radix and Ganoderma lucidum ) enhance im- mune response of carp, Cyprinuscarpio, and protection against Aeromonas hydrophila [ J]. Fish Shell fish Immunology,2008,275 : 26 - 33.