瘦素受体在子宫肌瘤组织中表达的初步研究

Expression of leptin receptor in leiomyomas

目的 测定瘦素受体在子宫肌瘤组织中的表达 ,探讨瘦素及其受体与子宫肌瘤的相关性。方法 采用逆转录聚合酶链反应 (RT PCR)技术 ,检测 4 3例全子宫切除手术子宫肌瘤组织及远离肌瘤的平滑肌组织中瘦素受体mRNA表达水平。同时采用放射免疫法 (RIA)检测子宫肌瘤病人及 4 0例正常对照组血清中瘦素水平。结果 子宫平滑肌及子宫肌瘤组织均存在瘦素受体mRNA表达 ,且表达水平无显著性差别 (P >0 .0 5 ) ,子宫肌瘤组 (增生期 )血清瘦素为 (1 1 .2± 3.8)ng/ml与对照组 (9.3± 4 .6 )ng/ml比较无显著性差异 (P >0 .0 5 )。结论 子宫肌瘤组织与子宫平滑肌相同有瘦素受体表达 ,瘦素。

Objective To investigate the relationship between leptin level and expression of leptin receptor and uterine leiomyoma. Methods The concentration of serum leptin of 43 complete hysterectomy women with leiomyoma was detected by radioimmunoassay (RIA). Reverse transcription polymerase chain reaction (RT PCR) was to examine leptin receptor mRNA expression in the uterine leiomyoma tissue. Results The leptin receptor mRNA showed no significant difference between uterine leiomyomas and normal uterine muscle tissue ( P >0.05). There was some obvious relation between leptin levels and expression of leptin receptor and the menstrual phase ( P <0.05). Conclusion These studies demonstrate expression of leptin receptor in human uterine leiomyomas and normal uterine muscle tissue. It could have no relationship among leptin and leptin receptor and leiomyoma developing.