葡糖基转移酶多肽疫苗HDS免疫大鼠的实验研究

The Study of Peptide Vaccine HDS from Streptococcus mutans Glucosyltransferase

目的 动物体内研究葡糖基转移酶多肽疫苗HDS免疫原性和防龋效果。方法 构建大鼠人工龋模型 ,分别用人工抗原HDS_KLH ,多肽抗原HDS ,葡糖基转移酶 (GTF)免疫大鼠 ,酶联免疫吸附法检测大鼠血清和唾液中抗HDS、GTF抗体 ;体视显微镜下观察各组大鼠磨牙龋坏情况 ,并用Keyes计分法计分。结果 HDS_KLH免疫组大鼠唾液、血清中抗HDSIgG和IgA水平均高于对照组 (P <0 0 5 ) ;HDS_KLH ,HDS ,GTF免疫组大鼠龋Keyes计分均低于对照组 (P <0 0 1) ,光滑面防龋效果最为显著。结论 人工抗原HDS_KLH能刺激机体产生针对GTF的保护性免疫 ,并减少实验大鼠龋齿发生。

Objective\ To investigate the antigenicity of the peptide vaccine HDS from Streptococcus mutans glucosyltransferase and its ability to induce protective immune responses in an experimental rat model of dental caries.Methods\ Artificial antigen HDS_KLH, peptide HDS, glycosyltransferase were injected to immunize rats. Measurement of the specific anti_HDS,GTF IgG or IgA concentration in saliva and serum were undertaken by ELISA among the experimental groups. Gnotobiotic rat model was developed when challenged S.mutans and a caries promoting diet. The jaws of the rats were selected and dyed. The Keyes caries score for each jaw were counted.Results\ The level of serum and salivary specific anti_HDS IgG and IgA in the group immunized by HDS_KLH was significantly higher than that in control group (P<0 05). The Keyes caries score of GTF,HDS and HDS_KLH immunized group were significantly lower than that of control group, especially lower in smooth tooth surface.Conclusion\ Artificial antigen HDS_KLH could induce immune response. As a peptide vaccine, HDS_KLH could reduce the caries incidence in experimental rat model.