甲壳动物血清中凝集素对微生物细胞的凝集性能

THE PROPERTIES OF AGGLUTINATION ON MICROBIAL CELLS BY LECTINS EXTRACTS FROM CRUSTACEAN SERUMS

用12种甲壳动物血清凝集素提取液 ,对10种微生物细胞进行凝集试验 ,发现每种甲壳动物血清至少能凝集3种微生物细胞 ,酿酒酵母 (Saccharomycescerevisiae)细胞对凝集素反应最为敏感。7种甲壳动物血清凝集素对酿酒酵母细胞的凝集可被2种以上的糖所抑制 ,12种甲壳动物血清凝集素的温度和 pH适应范围表现不一 ,但它们的凝集活性都被EDTA所抑制。

Twelve species of crustaceans were detected for agglutination using ten kinds of microbial cells.It showed that serum extracts fromtwelve species of crustacean were able to agglutinate at least more than three kinds of microbial cells,and the cell of Saccharomyces cerevisiae was the most sensitive to agglutination made by serums.The activity of seven crustacean serums agglutinating on cell of S.C.were inhibited by two or more kinds of saccharides.And the agglutinative activities of12crustaceans on the cell of S.C.were different in the range of pH4.0～10.0and in the range of temperature4～90℃,but their agglutinative activities were inhibited by EDTA.