乙型肝炎患者外周血细胞中Th1/Th2型细胞因子的表达及其临床意义

Th1/Th2 type cytokines expression in peripheral blood cell of the patients with hepatitis B virus infection and its clinical significance

目的 :探讨Th1 Th2型细胞因子在HBV感染的临床多种表现形式中的表达特征及其临床意义。方法 :采用四色法流式细胞术检测乙型肝炎患者和正常人外周血细胞中CD3+CD8+、CD3+CD8-细胞内的IFN γ和IL 4的表达情况。结果 :与正常对照组相比 ,急性乙肝患者的Tc2细胞百分比增高 ,慢性乙肝患者的Th1、Tc1细胞百分比减低 ,也低于急性乙肝患者 ,但Th1、Tc1细胞百分比随着慢性乙型肝炎肝脏炎症活动的加剧而逐渐增多。分泌IL 4的Th、Tc细胞在正常人和各组病例中无显著性差异。结论 :急慢性乙肝患者的Th、Tc细胞均以分泌Th1型细胞因子占优势 ,但数量的差异可能是HBV感染者不同结局的原因之一。

Objective:To explore the Th1/Th2 type cytokines expression and its clinical significance in various clinical behaviors of HBV-infected individuals.Methods:The production of IFN-γ and IL-4 by CD3+CD8+?CD3+CD8-cells in peripheral blood from normal donor and patients with hepatitis B virus infection were assessed by a four-color flow cytometry.Results:Compared with the healthy control,the percentage of Tc1 cells in AHB group was higher,whereas the percentage of Th1 and Tc1 cells in CHB group were lower,and lower than that of the AHB,but they increased significantly with the chronic hepatic inflammation activity.Conclusion:T-helper 1 type cytokines are predominant in peripheral blood cell of AHB and CHB,but the different percentage of Th1 type cytokines may be one reason of the final outcome of the HBV-infected individuals.