干扰素对HGV和HCV重叠感染中HGV的疗效研究

Curative effect Of interferon therapy to HGV in patients with HGV and HCV confection.

本研究通过探讨干扰素(IFN)对庚型肝炎病毒(HGV)和丙型肝炎病毒(HCV)重叠感染的慢性肝炎患者中HGV的疗效,以明确HGV感染的临床意义。利用逆转录多聚酶链反应(RT-PCR),并对其产物直接进行测序。在IFN治疗期间,91.1％患者血中的HGV转阴。只有26.8％患者持续阴性。血中ALT水平在停用干扰素后与HCV-RNA水平密切相关,而与HGV-RNA的水平无明显关联。(1)HGV对IFN治疗敏感;(2)在HGV与HCV重叠感染的丙型肝炎患者中,HCV对肝细胞的损伤起重要作用,而HGV直接导致肝损伤且使肝损伤进展的可能性很小。

In the present study, we examined the effect of interferon (IFN) on the HGV in chronic hepatitis C patients who were coinfected with HGV, in order to clarify the clinical significance of HGV infection. HGV - RNA detection, RT - PCR with primers from the 5' - UTR was used for HGV - RNA quantitative analysis, competitive RT - PCR was employed. Products of PCR were directly sequenced. During IFN therapy, serum HGV- RNA became negative in 91.1% However, a breakthrough of HGV was seen in 20% , a virological relapse was seen after the cessation of IFN therapy in 40% , and only 26.8% were sustained as negative. The changes in serum ALT levels after the cessation of IFN therapy correlated well with the changes in HCV - RNA. However, the correlation with HGV- RNA was poor. (1)HGV is sensitive to IFN therapy; (2)In chronic hepatitis C patients coinfected with HGV and HCV, HCV appears to the involved with hepatocellular injury. It is less likely that HGV is directly involved in the initiation and progression of hepatic injury.