^(188)Re直接标记牛血清白蛋白的方法学研究

Investigations of directly labeling BSA with Rhenium- 188

目的探索具有较高放化纯度和良好稳定性的188Re直接标记牛血清白蛋白的方法。方法通过改变络合剂、缓冲液、标记反应时间、pH值、淋洗液体积、氯化亚锡的用量、反应温度等各项标记条件来探讨最佳标记条件。结果188Re直接标记牛血清白蛋白最佳标记条件:0.1ml葡萄糖酸钠溶液(0.3mol/L);0.08ml浓盐酸溶解的SnCl2·2H2O(10mg/ml);0.04ml0.2mol/L乙酸缓冲液(pH值5.0);0.04ml牛血清白蛋白(10mg/ml);188ReO4-淋洗液0.1ml。188Re直接标记牛血清白蛋白放化纯度达(96.8±1.06)%,标记反应中胶体含量均小于10%。结论预锡化直接标记法简便快速,能够得到较高的放化纯度,稳定性好,无需进一步纯化。

Objective To establish a useful and stable method for direct labeling of bovine serum albumin (BSA) with 188Re. Methods The ' pretinning' procedure was a BSA. The labeling conditions, such as chelate agent, buffer, the pH value of acetate buffer, the volume of 188Re perrhenate, the amount of SnCl2, reaction temperature, were changed. Results The optimum conditions were 0.1ml sodium gluconate (0.3mol/L), 0.08ml stannous chloride (10mg/ml), 0.04mL acetate buffer (pH 5.0), 0.04ml BSA (10mg/ml), 0.1ml 188Re perrhenate, incubation on room temperation. It was found that the radiochemical purity of 188Re BSA could reach (96.8± 1.06)% and the amount of radiocolloid was less than 10% . Conclusion This method of directly labeling 188Re to BSA with SnCl2 and sodium gluconate is stable and the high radiochemical purity was obtained. [