诱导免疫耐受抑制小鼠异体坐骨神经移植排斥反应的实验研究

Immunotolerance-inducing inhibits transplantation rejec tion of ischiadic nerve allograft in mice

目的 探讨胸腺内注射异基因抗原对异体鼠坐骨神经移植存活的作用。方法 自供体C5 7BL 6小鼠脾细胞中提取MHC抗原 ,注入受体Balb c小鼠胸腺内 ,2周后移植供体C5 7BL 6小鼠坐骨神经。同时另设 4组对照组 ,即自体神经移植组 ,异体神经移植组 ,冷冻后异体神经移植组 ,应用免疫抑制剂异体神经移植组 ;3周后进行神经电生理检查、组织学的检测。结果 实验组优于异体神经移植组及冷冻后异体神经移植组 ;接近于自体神经移植组及应用免疫抑制剂异体神经移植组。结论 胸腺内注射供体MHC抗原可诱导特异性坐骨神经移植耐受。

Objective To study the effection of intrathymical injection allog enic antigens on survive time of ischiadic nerve allograft. Methods MHC antigen extracted from splenic cells of donor C57BL/6 m ice was intrathymically injected to recipients adult Balb/c mice,donor C57BL/6 i schiadic nerve was transplanted after 2 weeks. In the same time,four control gro u ps was used,such as nerve autograft group,nerve allograft group,nerve freeze tha wing graft group and nerve allograft in use of immunosuppressant group. The elec trophysiological and histological changes of the graft were observed after 3 wee ks. Results The test group surpassed nerve allograft group,nerve freeze thawing graft group and approached to autograft group and nerve allograft in u se of immunosupressant group. Conclusion The intrathymical injection of donor MHC antigen could i nduce specific immunotolerance of ischiadic nerve transplantation.