Vitamin D3对乳腺癌荷瘤裸鼠的治疗研究

Vitamin D3 for the treatment of breast cancer:a study of Balb/c nude mouse model

目的探讨维生素D3(vitaminD3,VitD3)对乳腺癌裸鼠移植模型的治疗作用。方法裸鼠皮下接种MCF 7乳腺癌细胞 ,建立乳腺癌裸鼠移植模型。用VitD3和三苯氧胺 (tamoxifen ,TAM )给荷瘤裸鼠用药。用药 4周后 ,检测肿块大小、血清钙、磷水平 ,并用流式细胞仪检测细胞凋亡和细胞周期。结果VitD3治疗组血清钙水平高于对照组 ,血磷水平低于对照组 ,VitD3使肿瘤细胞凋亡增加 ,并使细胞停留在G0 G1期 ,与TAM有协同作用。结论VitD3和TAM可明显诱导乳腺癌细胞凋亡 ,并阻滞细胞停留在G0 G1期。

Objective To study the effect of vitamin D3 on breast cancer xenografted on a nude mouse model. MethodBalb/c nude mouse was injected subcutaneously with 10 6 human breast cancer cell line MCF-7.Two thousand units Vitamin D3 was administered in mice i·m every the other day. Tamoxifen (5 mg/kg) was fed per mouse daily. Mice were divided into four groups with ten mice in each group: Vitamin D3 group, Vitamin D3 and tamoxifen group, tamoxifen group and control group. After 4 weeks, mice were killed to measure volume of tumor and calcium and phosphorus levels in serum. Tumor cells were analyzed with flow cytometery to detect apoptosis and cell cycle. Results Calcium levels in serum of mice treated with vitamin D3 were higher than that of other two groups, but phosphorus levels in serum were lower . Apoptosis rate was higher than that of control group and tumor cells were arrested in G0/G1 phase, especially those treated with vitamin D3 and tamoxifen. ConclusionsVitamin D3 could significantly induce apoptosis of breast cancer cells and arrest tumor cells in G0/G1 phase. It is synergic with tamoxifen and this may have clinical significance in the treatment of breast cancer.