应用等电聚焦电泳测定玉米种子的纯度

Determing Purity of Corn Seeds with Isoelectric Focusing Electrophoresis

应用灵敏度、分辨率和重复性均较高的聚丙烯酰胺凝胶等电聚焦(IEF)电泳测定150多种玉米自交系和90多种玉米杂交种的胚及幼芽的酯酶(EST)同工酶,通过对酶谱的比较和遗传分析,发现它们的酶谱各有特点,而且相互间又有很显著的差异,这些差异正是它们基因型差异的反映。因此,酶谱的特点及差异为玉米自交系和玉米杂交种种子纯度的鉴定从遗传和生化角度提供了科学依据。

Esterase isozymes in more than 150 corn inbred lines and 90 corn hybrids were investigated using polycrylamide gel isoelectric focusing electrophoresis. All of the inbred lines and hybrids could be unequivocally distinguished from one another by their zymograms. According to the zymogram,the purity of the specific corn inbred or hybrid could be counted and showed by the percentage.The germination tests were taken at the same time in the field by using the same com inbred and hybrid.The two tests showed the same results. IEF is a very useful quick and accurate method to determine the purity of corn inbred and hybrid and is widely used in corn breeding program and com production.