摘要
目的 观察非甾体抗炎药所致肠道损伤时口服及喷雾给予鸡Ⅱ型胶原 (CⅡ )对大鼠佐剂性关节炎 (AA)的治疗作用 ,并比较鸡CⅡ 2种制剂疗效的差异。方法 在建立大鼠AA模型的基础上 ,以 15mg/kg的美洛昔康诱导AA大鼠肠炎 ,给予不同剂型鸡CⅡ (2 0 μg/kg) ,连续 7d。 结果 致炎后 2 0d ,鸡CⅡ喷雾组对AA大鼠的继发性炎症有明显的抑制作用。致炎后第 2 4天 ,鸡CⅡ灌胃 (ig)组作用出现 ,至第 2 8天时鸡CⅡig组作用消失 ,鸡CⅡ喷雾组仍对AA大鼠的继发性炎症有抑制作用。粪便隐血 (OB)检验结果和髓过氧化物酶 (MPO)水平均高于正常组 ,表明模型复制成功。鸡CⅡ能显著逆转肠道损伤 ,降低升高的MPO和OB检验结果。结论 鸡CⅡ对伴有肠道损伤的AA大鼠具有明显的治疗作用 。
Objective To investigate the therapeutical ef fe ct of chicken collagen typeⅡ (CⅡ) in adjuvant arthritis (AA) rats with intesti nal injuries,and the intestinal injuries were caused by NSAIDs. Intragastric ad ministration or spray administration of CⅡwas given. The different effects of t hese two angents were comparped. Methods On the basis of establis hment of AA model, we made the enteritis model with meloxicam (15 mg/kg). Two an gents of CⅡ(20 μg/kg) were given seven days on end. Results In spray group, significant inhibition of secondary inflammation was observed on t he 20th day after CⅡ administration and this effect continued to be observable on to the 28th day. In ig group, however, the inhibition was only observable on the 24th day, and disappeared on the 28th day. The indicator of occult blood test and the level of myeloperoxidase were all higher than that in normal group. These data showed the model was established s uccessfully. We used CⅡ to treat the rats, and found that it reversed intestine s damage, reduced occult blood and the level of myeloperoxidase. Conc lusion The results suggest that CⅡ has therapeutical effect in AA rats, and the effect of spray administration of CⅡ is better than intragastric admin istration of CⅡ.
出处
《安徽医科大学学报》
CAS
2003年第5期360-363,共4页
Acta Universitatis Medicinalis Anhui
