萝卜组培苗与实生苗若干生理生化性状的比较研究

Physiological and Biochemical Differences between Regenerated Plants and Seedlings of Radish (Raphanus sativus L. )

对萝卜组培苗与实生苗生长过程中若干生理生化性状进行了研究,结果表明,组培苗叶片中可溶性糖含量高于实生苗,而根部可溶性糖含量低于实生苗;组培苗叶片中过氧化物酶(POD)活性在定植后42d内低于实生苗,56d时显著增加并高于实生苗,之后又回落,70d时低于实生苗;而组培苗根部POD活性始终高于实生苗;组培苗叶片和根中超氧化物酶(SOD)活性均高于实生苗。组培苗和实生苗的酯酶(EST)、POD及淀粉酶同工酶酶谱在叶片与根部之间有差异,但相同部位均没有出现特征带。

Physiological and biochemical investigation of the differences between regenerated plants and seedling plants were conducted in radish (.Raphanus sativus L. ). During the whole plant development, soluble sugar content was higher in leaves of regenerated plants than in those of seedling plants, whereas lower in roots of regenerated plants than that of seedling plants . Activity of POD in leaves of regenerated plants was much higher than in those of seedling plants at the development stage of 56 d, and then began to descend until it was lower than that of seedling plants on 70 d. Activity of POD in roots of regenerated plants was higher than those of seedling plants throughout the plant development. Activity of SOD in regenerated plants was higher than seedling plants both in leaves and roots in most time of the growth period. The isoenzymes of EST, POD and amylase at different development stages were studied by means of polyacrylamide gel electrophoresis. The differences exist in each enzymic pattern of polyacrylamide gel electrophoresis, but there was no specific band either in regenerated plants or in seedling plants. These findings yield insights into the mechanism of the root formation of radish.