用比布列西猩红共振光散射技术测定蛋白质(英文)

Determination of Proteins with Biebrich Scarlet by a Resonance Light Scattering Technique

在酸性溶液中,比布列西猩红与蛋白质作用,产生共振光散射(RLS)增强光谱,最大散射峰位于286 0nm,且增强RLS强度在一定范围内与蛋白质浓度呈线性关系.研究了pH、比布列西猩红的浓度及离子强度对RLS强度的影响.人血清白蛋白(HSA)的线性范围为0 02～4 0μg/mL,牛血清白蛋白(BSA)为0 02～4 5μg/mL,溶菌酶(Lys)为0 02～2 0μg/mL,γ 球蛋白(γ IgG)为0 01-7 5μg/mL.测定的检测限(3σ)分别为:16 6ng/mL(HSA),15 7ng/mL(BSA),16 7ng/mL(Lys)和7 5ng/mL(γ IgG).此方法用于人血清样品测定,结果满意.

In acidic solution, the interaction of Biebrich Scarlet (BS) with protein results in strong enhanced resonance light scattering (RLS) signals characterized by peak at 286.0 nm. It was found that the enhanced RLS intensities were in proportion to the concentrations of proteins in a certain range. The effects of pH, BS concentration and ionic strength on binding interaction were investigated. The linear ranges of human serum albumin (HSA), bovine serum albumin (BSA), lysozyme (Lys) and γ-globulin (γ-IgG) were 0.02-4.0 μg/mL, 0.02-4.5 μg/mL, 0.02-2.0 μg/mL and 0.01-7.5 μg/mL, respectively. The detection limits (3σ) were 16.6 ng/mL for HSA, 15.7 ng/mL for BSA, 16.7 ng/mL for Lys and 7.5 ng/mL for γ-IgG, respectively. Human serum samples were measured satisfactorily by using this method.