摘要
采用紫外区域内对底物的扫瞄法研究了高灌蓝越橘(VaccyniumcorybosumL.)多酚氧化酶的底物专一性,用紫外分光光度法和极谱测氧消耗法对酶的动力学进行了研究,测定了相应的米氏常数。用二类抑制剂研究抑制剂浓度对抑制剂效果的影响以及抑制剂与酶结合不同时间对抑制效果的影响,从而对高灌蓝越橘多酚氧化酶进行了抑制研究。
The substrate specificity of highbush blueberry polyphenol (Vaccyn iu m corybosum L.) oxidase has been studied by a substrate scanning method in spec trophotometric range. The enzymeatic kinetics has been investigated by using th e spectrophotometric method and poloragraphe method. Michaelis-constant(Km) has been assayed. The kinds of inhibitors and the effects of inhibitor concentrati o n and of combination time of inhibitor and enzyme on the inhibition of PPO hav e also been stadied.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2003年第4期55-58,共4页
Food Science
