摘要
采用糖腈乙酸酯气相色谱法,利用OV-1701毛细管色谱柱完成了7种单糖的糖腈乙酸酯及内标肌醇六乙酰酯的分离,测定了灰树花多糖粗品及4种级分的单糖组成。方法的平均回收率在98.5%～101.6%之间,相对标准偏差低于2%。
The separation and determination of monosacchride composition in cude polysaccharide and polysaccharide fractions from Grifola frondosa is reported by gas chromatography with OV-1701 capillary colume. Monosacchrides from polysacc harides hydrolyzed with 2.0mol/L CF3COOH are reduced and derivatized to sugar ac etylated aldononitriles before detemination. The linear correlation coefficients of 7 monosaccharides were all over 0.997, the recoveries of monosaccharides ran ged 98.5%～101.6%, and the relative standard deviations were below 2%.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2003年第4期110-113,共4页
Food Science
基金
湖北省"十五"重点科技攻关计划资助项目(2001AA204B03)
