连续51天服用褪黑激素对小鼠肾脏、心肌与脑部分生化指标及体重变化的影响

Effects of Continuous Administration of MT during 51 days on Chemical Indexes in Mice's Kidney,Heart and Brain and the Growth of Weight

观察连续51d服用褪黑激素(MT)对机体抗氧化系统及体重增长的影响。方法:以雄性昆明种小鼠为实验对象,连续51d服用MT建立动物模型。分别测试肾脏、心肌与脑超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性、过氧化氢酶(CAT)活性、丙二醛(MDA)含量、总抗氧化能力(T-AOC)及整个服用期间的体重变化情况。结果:连续51d服用MT后,MT组小鼠体重及体重增量明显小于对照组(p<0.05)。小鼠肾脏SOD活性与T-AOC,心肌T-AOC及脑SOD活性与T-AOC显著升高(p<0.05～0.01),脑CAT活性及肾脏、心肌与脑MDA含量显著下降(p<0.01),但对肾脏POD活性与CAT活性,心肌SOD活性、POD活性与CAT活性以及脑POD活性无显著性影响(p>0.05)。结论:MT具有很强的抗氧化能力,对机体的抗氧化系统有明显的辅助作用,对小鼠体重的正常生长发育存在一定程度的抑制。

Objective:To investigate effects of the continuous administration o f Melatonin during 51 days on the anti-oxidation system and the growth of weigh t. Methods: The objects were Kunming mice (♂). The model was set up by continu o us administration of MT during 51 days. The indexes include the activity of Su pe roxide Dismutase(SOD) , Peroxidase(POD), Catalase(CAT),Total anti-oxidant Ca pab ility(T-AOC) and weight. Results: After continuous administration of MT, mi ce′ s weight and the increment of weight are significantly lower in MT group th an in control group(p< 0.05). Continuous administration of MT can significantly enhan ce the activity of SOD and T-AOC in kidney, T-AOC in heart, SOD and T-AOC in brain (p< 0.05～0.01),and significantly decrease the activity of CAT in brain an d the content of MDA in kidney, heart and brain(p< 0.05～0.01), but ins ignifican tly effect on the activity of POD and CAT in kidney, SOD, POD and CAT in heart a nd POD in brain(p >0.05). Conclusion: Experimental results suggest th at the stro ng anti-oxidation capability of MT can significantly assist anti-o xidation sys tem of body, and partly inhabit the growth of weight.