HBV核酸光敏生物素探针的制备

The Preparation of HBV Nucleic Acid Photoactive Biotin Probe

利用设计合成的特异性引物,通过PCR扩增获得乙肝病毒的S区621个bp DNA片段。该扩增产物经EcoRI与BamHI双酶切后直接克隆进入PSK载体进行表达,再通过扩增重组菌株,以获得大量的S区DNA片段,对它们进行光敏生物素标记,制成探针,对乙肝病毒进行检测灵敏度高,特异性强,其灵敏度可达到4pg水平。

To utilize the synthesized idiosyncratic primer, the 621 bpDNA fragments in S-district of HBV can be obtained by the amplification of PCR. After the product is cut by enzyme of EcoRI and BamHI, it is directly cloned and expressed in the PSK vehicle, then is amplified into the recombined strain, to obtain many fragments of DNA in S-district of HBV. If they are marked with photoactive biotin and become probe, it will be high sensitive and strongly idiosyncratic to detect the HBV. The sensitivity can reach 4pg level.