鼻咽癌患者血清中活性氧、超氧化物歧化酶和谷胱甘肽过氧化物酶活性测定

Express of plasma ROS, SOD and GSH-PX in patients with nasopharyngeal carcinoma

目的 :测定鼻咽癌患者血清中活性氧 (ROS)、超氧化物歧化酶 (SOD)和谷胱甘肽过氧化物酶 (GSH PX)的活性水平 ,以探讨鼻咽癌患者体内自由基代谢状态及其在鼻咽癌发生、发展中的作用。方法 :分别采用Fenton反应显色法 ,黄嘌呤氧化酶法和二硫代二硝基苯甲酸法对 4 0例鼻咽癌患者 (鼻咽癌组 )和 2 0例健康体检者 (对照组 )血清中ROS、SOD和GSH PX水平进行检测。结果 :鼻咽癌组血清中ROS活性 [(11 32 7.835±4 83.777) /(U·ml-1) ]显著高于对照组 [(10 2 6 2 .712± 5 2 5 .2 87) /(U·ml-1) ](P <0 .0 5 ) ,SOD和GSH PX活性[(76 .6 19± 2 2 .2 83) /(NU·ml-1) ,(98.6 5 3± 4 6 .374 ) /AU ]显著低于对照组 [(15 4 .6 0 3± 2 7.2 4 1) /(NU·ml-1) ,(30 7.872± 116 .2 75 ) /AU](P <0 .0 5 ) ;不同病理类型鼻咽癌患者血清中ROS、SOD和GSH PX活性间的差异均无显著性意义 (P >0 .0 5 )。结论 :鼻咽癌患者体内自由基代谢紊乱 ,抗氧化损伤能力显著下降 。

Objective:To investigate the levels of plasma reactive oxygen species( ROS), superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX ) in patients with nasopharyngeal carcinoma ( NPC ) and to observe the state of metabolism of free radical in patients with NPC and the effects of them on nasopharyngeal carcinoma.Method:The levels of plasma ROS, SOD and GSH-PX in 40 patients with NPC and 20 normal controls were identified by Fenton reaction method, xanthine oxidase method and dithiocarbamate method respectively.Result:Plasma ROS activity[( 11327.835± 483.777)/(U·ml -1)] in NPC increased significantly in comparison with the control group [( 10262.712± 525.287)/(U·ml -1)](P< 0.05). Plasma SOD and GSH-PX activities [( 76.619± 22.283)/(NU·ml -1), ( 98.653± 46.374)/AU] in NPC decreased significantly in comparison with the control group [( 154.603± 27.241)/(NU·ml -1),( 307.872± 116.275)/AU] (P< 0.05). But there was no significant difference of the levels of plasma ROS[( 10987.235± 469.340)/(U·ml -1),( 10081.53± 488.409)/(U·ml -1),( 12085.044± 564.721)/(U·ml -1)],SOD[( 79.517± 20.368)/(NU·ml -1),( 67.975± 24.202)/(NU·ml -1),( 58.692± 29.946)/(NU·ml -1)] and GSH-PX[( 101.362± 59.859)/AU,( 89.338± 42.259)/AU,( 101.890± 33.559)/AU] in different pathological kinds of NPC.Conclusion:The metabolism of free radical is disturbed and the ability of anti-oxidation injury decreases significantly in patients with NPC, so anti-oxidation treatment should be given to suppress the development of NPC.