c-Ha-ras基因3′端可变串联重复序列位点遗传多态性与葡萄胎的恶变关系

Polymorphism of c-Ha-ras-3′-VNTR in hydatidiform mole a nd its relationship with the development of postmolar tumor

目的 探讨遗传标记物 c-Ha-ras基因 3′端可变串联重复序列 (variable numbers of tandemrepeats,VNTR)在妊娠滋养细胞疾病中的变化 ,以了解 DNA来源与葡萄胎恶变的关系。方法 用扩增片段长度多态性 -聚丙烯酰胺凝胶电泳方法对 c-Ha-ras基因 3′端 VNTR位点进行分析 ,将患者病变组织与其父母的 DNA扩增结果进行对照 ,以判定病变组织 DNA的来源。结果 在 15组病例中 ,仅有 2例葡萄胎组织的 DNA扩增片段全部来自父方 ;既来自父方又来自母方者有 13例 ,其中 2例来自父母双方的比例是一样的 ,11例来自父方的比例大于来自母方的比例。结论 DNA来自父母亲双方的 。

Objective: To study the polymorphism of c-Ha-ras-3′-variable number of tanden repeat (VNTR) and its relationship with development of postmolar tumor. Methods: The cases of hydatidiform mole(HM) were retrospectively analyzed by amplified fragment length polymorphism-polyacrylamide gel electrophoresis. The DNA origin of HM was determined by comparison with the parents' DNA amplified results. Results: Among the samples from 15 cases, DNA from only paternal origin was found in 2 cases. DNA from both parents was in 13 cases, and of these 13 cases, 2 were found to be had balanced DNA origin, 11 had more DNA form paternal origin than DNA from maternal origin. Conclusion: The HM which has DNA from both parents origin and predominantly from paternal origin developed to postmolar tumor more frequently.