正畸移动狗乳磨牙后其恒牙胚牙周组织CSF-1及TGF-β1的表达及意义

CSF-1 and TGF-β1 Expression in periodontal tissue of third premolar germ during second primary molar orthodontic movement in dog.

目的 :研究狗乳牙正畸移动后其恒牙胚牙周组织中集落刺激因子 (CSF 1)及转化生长因子 β1(TGF β1)的表达 ,探讨乳牙移动后其恒牙胚改变的机制。 方法 :取狗下颌第二乳磨牙正畸移动后 14d的组织标本 ,用免疫组织化学染色技术观察TGF β1和CSF 1在恒牙胚牙囊、牙周牙槽骨组织中的表达。结果 :TGF β1和CSF 1的染色在乳磨牙牙根的远中牙周膜和牙槽骨中以及恒牙胚的远中牙囊和牙槽骨中的表达明显减弱 ,在乳磨牙牙根的近中牙周膜和牙槽骨中以及恒牙胚的近中牙囊和牙槽骨中的表达明显增强。结论

Objective: To investigate the expression of CSF-1 and TGF-β1 in periodontal tissue of third premolar germ during second primary molar orthodontic movement in dog. Methods: Orthodontic appliance with the force of 0.784 N for the mesial movement of mandible second primary molar was used in 4 dogs for 14 d.Then the samples including mandibular second and third molars and surrounding tissues were prepared for CSF-1 and TGF-β1 detection by immunohistochemical staining technique. Results: Strong CSF-1, TGF-β1 expression was found in the dental follicle and alveolar bone of mesial part of third premolar germ, and weak in the dental follicle and alveolar bone of distal part of third premolar germ in the treated side of the dogs. Conclusion: CSF-1 and TGF-β1 may play roles in the bone remodeling for orthodontic primary tooth movement.