微波辐照对鼠脑MDA、NOS含量和LDH活性的影响

Effects of Microwave on the Contents of MDA and NOS, Activities of LDH in Brain of Mice

为研究手机微波对小鼠脑是否有损害 ,选用 4种不同频率和不同发射功率的微波照射小鼠 ,用TBA比色法、二硝基苯肼显色法 (DNPH显色法 )和吩嗪二甲脂比色法 (PMS显色法 )检测小鼠脑组织中脂质过氧化产物丙二醛(MDA)、乳酸脱氢酶 (LDH)和一氧化氮合酶 (NOS)。统计学分析显示 :2 45 0MHz微波辐射后MDA含量升高 (P <0 .0 0 1 ) ,LDH活性降低 (P <0 .0 5 ) ,NOS不变 (P >0 .0 5 ) ;870、45 0和 45MHz微波分别辐照后 ,MDA含量和LDH活性均变化不大 (P >0 .0 5 )。提示 :在强辐照条件下 (2 45 0MHz) ,微波通过使脑中自由基产生过量参与脑损伤 ;而在手机弱辐照条件下 (870、45 0、45MHz) ,微波辐照对脑基本无损伤 。

To research if there is injury caused by microwave of hand-set in the brain of mice, microwaves of four kinds of different frequencies and shooting power were used; TBA, DNPH and PMS were used to determine the contents of MDA and activities of LDH and NOS in the brain of the mice after microwave exposure. The contents of MDA and activities of LDH did not change(P>0.05) after weak microwave(870, 450 and 45 MHz, 100 mW/cm 2) exposure with hand-set and this proved hand-telephones were safe. The content of MDA in the brain of mice increased(P< 0.001), the activity of LDH decreased(P<0.05) and the activity of NOS did not change(P> 0.05) after strong microwave exposure (2 450 MHz, 730 mW/cm 2), the reactive oxygen free radicals in the brain of the mice was produced after strong microwave (2 450 MHz, 730 mW/cm 2) exposure and this indicated the oxygen free radicals may participate in the injury in the brain of the mice.