聚乙二醇/聚对苯二甲酸丁二醇酯多孔细胞支架的制备与表征

Preparation of Porous cell Scaffolds of Poly(ethylene glycal/butylene terephthalate)

以聚乙二醇 /聚对苯二甲酸丁二醇酯 (PEG/PBT)嵌段共聚物为原料 ,采用溶液浇铸 颗粒滤除法制备了在不同致孔剂粒子尺寸条件下的一系列PEG/PBT多孔细胞支架 ;用质量法测定其孔隙率 ;并在PEG/PBT多孔支架上进行细胞培养。研究结果表明 :随着制备过程中致孔剂用量的增加 ,多孔支架的孔隙率逐渐增加 ,致孔剂的颗粒大小对多孔支架的孔径影响较大 ,对孔隙率影响较小。在致孔剂用量及致孔剂颗粒尺寸都相同的情况下 ,随着共聚物中PBT的增加 ,孔隙率逐渐下降 ,细胞 (培养 )在支架上繁殖情况良好 。

The highly porous biodegradable cell scaffolds of poly(ethylene glycal/butylene terephthalate)were prepared with a solution-casting and porogen particular-leaching technology. A method of a graduated cylinder was used to measure the porosity of the porous scaffold. It was found that the porosity of the porous scaffold increased with the porogen weight fraction, independent of porogen particle sige. As the content of the butylene terephthalate copolymer increased, the porosity of the porous PBT scaffold decreased. The median pore diameter depended on the sige of porogen particle. It was also found that the cartilage proliferated in the PEG/PBT scaffolds and secreted extracellular matrix(ECM).