人眼睫状肌细胞的培养与鉴定

Culture and Identification of Human Ciliary Muscle Cell

为了提供从细胞水平研究葡萄膜、巩膜途径房水引流的实验材料 ,分离死亡后 8～ 2 0h眼库角膜取材后的人眼睫状肌组织 ,用D MEM进行培养 ,用光镜、电镜、免疫荧光法分别进行鉴定。发现 :光镜、电镜下培养的细胞具有典型的睫状肌形态和结构 ,Smoothmuscleα actin染色呈强阳性 ,Desmin染色呈弱阳性。提示 :选择死亡后 8～ 2 0h的睫状肌组织用D

To provide experimental material for study of uveoscleral outflow. Material and methods: ciliary muscles were isolated 8～20 h after death, cultured with D-MEM, identified by microstructure and immunofluorescence. Typical ciliary muscle cell structure was observed under hight microscope and electronical microscope. The stain of anti smooth muscleα-actin and anti desmin were strongly positive and weakly positive respectively. Ciliary muscle cell can be cultured by this kind of method.