滋补肝肾通络解毒中药对帕金森病大鼠旋转行为的影响

Effect of Chinese herbs of nourishing liver & kidney, clearing collaterals and detoxification on rotational behavior of rats with Parkinson's disease

目的:阐明滋补肝肾通络解毒中药治疗帕金森病的作用机制。方法:采用6-羟基多巴胺(6-hydroxydopamine,6-OHDA)注射于脑右侧黑质造成偏侧帕金森病(Parkinson'sdisease,PD)模型,并用滋补肝肾通络解毒中药进行治疗,同时设立正常对照组、假手术组,观察中药对PD大鼠旋转行为的影响。结果:正常对照组、假手术组在治疗前后均未发现动物有旋转行为。而模型组和中药组在治疗前两组的旋转圈数分别为269±99和253±50,差异无显著性意义(t=0.649,P>0.05);治疗后两组的旋转圈数分别为263±87和164±35,差异有显著性意义(t=4.748,P<0.01)。其中,与模型组比较,中药组的旋转圈数明显减少(t=9.544,P<0.01)。结论:滋补肝肾通络解毒中药能明显改善模型大鼠的旋转行为。

AIM:To illustrate the action mechanism for treatment of Parkinson's disease (P D) with Chinese herbs of nourishing liver and kidney, clearing collaterals and d etoxification. METHODS:Lateral PD model was established with injection of 6-hydroxydopamine into the black substances of the right-side brain, treated with Chinese herbs o f nourishing liver and kidney, clearing collaterals and detoxification. Simultan eously, normal control group and sham-operation group were set up to observe th e effect of Chinese herbs on rat's rotational behavior. RESULTS:There was no sinning behavior of rat in the control and sham-operatio n groups before and after treatment.The number of rotations was 269±99 for the model group and 253±50 for the Chinese herbs group before treatment, with no si gnificant differences (t=0.649,P >0.05), while the number of rotations was 263± 87 and 164±35 for the two groups after treatment and a difference was significa nt between the two groups (t=4.748,P< 0.01). Compared with the model group, the number of rotation in the Chinese herbs group decreased significantly (t=9.544,P < 0.01).