真皮支架聚乙交酯丙交酯海绵状膜的生物相容性研究

Study on the biocompatibility of sponge membrane made of polylactic a cid/polyglycolic acid copolymers

目的:检测聚乙交酯丙交酯(polylacticacid/polyglycolicacidcopolymers,PLGA)海绵状膜的生物相容性,探讨作为细胞支架的可能性。方法:将SD大鼠20只用随机数字表法分为5组,在其背部埋置PLGA海绵状膜。不同时相点取材,做组织学观察。用PLGA浸提液进行细胞毒性试验。结果:PLGA海绵状膜在体内两三周后有大量细胞长入并伴新生血管形成,炎性细胞较少。4周开始降解,8周完全降解。细胞毒性试验表明其细胞毒性为1级。结论:此种生物材料具有较好的生物相容性和可控的降解速率,可以作为细胞支架应用于组织工程研究。

AIM:To detect the biocompatibility of sponge membrane, which is made of polyla ctic acid/polyglycolic acid copolymers(PLGA) and to investigate the possibility of being used as cytoskeleton. METHODS:Twenty Sprague-Dawley rats were randomly divided into 5 groups at dig ital scale and their subcutaneous were buried with sponge membrane. Biopsies for histological analysis and cytotoxicity test of PLGA membrane were performed. RESULTS:Two to three weeks after implantation, there were large amounts of fib roblasts ingrowth within PLGA membrane companion new blood vessel development. T here were little inflammatory cells. The membrane begun to degrade about 4 weeks and degraded completely at 8 weeks. Its cytotoxicity was one degree.