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非SARS患儿体内SARS冠状病毒抗体的检测分析 被引量：1

Detection and analysis of SARS coronavirus-specific antibodies in sera from non-SARS children
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摘要 目的探讨非急性重症呼吸综合征(SARS)患儿体内是否存有SARS冠状病毒相关抗体。方法采用国家药品生物制品检定所正式检定通过的2种SARS抗体诊断试剂(间接免疫荧光检测试剂和双抗原夹心ELISA试剂),对广州地区1 060例非SARS患儿血清样本进行检测。结果1 060例非SARS患儿血清样本,间接免疫荧光法检测IgM、IgG均为阴性。双抗原夹心ELISA法检测也仅有2例出现弱阳性。结论非SARS患儿体内并未存在SARS病毒相关抗体。 Objective To examine the presence of severe acute respiratory syndrome (SARS) coronavirus-specific antibodies in the sera from non-SARS children. Methods Indirect immunofluorescent assay and double-antigen sandwich enzyme-linked immunosorbent assay (ELISA) were used to detect the virus-specific antibodies in sera of 1 060 non-SARS children in Guangzhou. Results All the serum samples from the 1 060 non-SARS children were negative for both IgG and IgM antibod-ies against SARS coronavirus as determined by indirect immunofluorescent assay, with only two serum samples showing weak positivity for SARS coronavirus-specific antibodies identified by double-antigen sandwich ELISA. Conclusion No SARS coronavirus-specific antibody are present in the sera of non-SARS children.
作者 李林海 石玉玲 李平 徐德兴 万根平 顾晓琼 张小玲 马清钧 曹诚
机构地区 广州军区广州总医院检验科 军事医学科学院生物工程研究所 广州市儿童医院检验科
出处 《第一军医大学学报》 CSCD 北大核心 2003年第10期1085-1087,共3页 Journal of First Military Medical University
关键词 小儿 SARS 冠状病毒 抗体 检测 急性重症呼吸综合征 severe acute respiratory syndrome (SARS), children antibody, SARS coronavirus/ analysis diagnosis reagent
分类号 R446.6 [医药卫生—诊断学]
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  • 1[3]Thomas G. Ksiazek, Dean Erdman, Cynthias G et al. A novel coronavirus associated with severe acute respiratory syndrome. The new Eng land Journal of medicine,2003,348(20) : 1 953
  • 2Stephensen CB, CaseBolt DB, Gangopadhyay NN. Phylogenetic analysis of a highly conserved region of the polymerase gene from 11 coronaviruses and development of a consensus polymerase chain reaction assay. Virus Res, 1999,60:181 - 189.
  • 3Vabret A, Mouthon F, Mourez T, Gouarin S, Petitjean J, Freymuth F. Direct diagnosis of human respiratory coronaviruses 229E and OC 43 by the polymerase chain reaction.J Virol Methods,2001,97:59-66.
  • 4Poutanen SM, Low DE, Henry B, Finkelstein S, Rose D, Green K,Tellier R, Draker R, Adachi D, Ayers M, Chan AK, Skowronski DM,Salit I, Simor AE, Slutsky AS, Doyle PW, Krajden M, Petric M, Brunham RC, McGeer AJ. Identification of severe acute respiratory syndrome in Canada. N Engl J Med. Low-1 - low-11 (at www.nejm.org on March 31,2003).
  • 5Ksiazek T G, Erdman D, Goldsmith C S, Zaki SR, Peret T, Emery S, Tong S, Urbani C, Comer JA, Lim W, Rollin PE, Dowell SF,Ling AE, Humphrey CD, Shieh WJ, Guamer J, Paddock CD, Rota P, Fields B, DeRisi J, Yang JY, Cox N, Hughes JM, LeDuc JW,Bellini WJ, Anderson LJ. A novd coronavims associated with severe acute respiratory syndrome. N Engl J Med, 2003,348:1953 - 1966.
  • 6Drosten C, Gunther S, Preiser W, van der Werf S, Brodt HR,Becker S, Rabenau H, Panning M, Koleanikova L, Fouchier RA,Berger A, Burguiere AM, Cinatl J, Eickmann M, Escriou N, Grywna K, Kramme S, Manuguerra JC, Muller S, Rickerts V, Sturmer M, Vieth S, Klenk HD, Osterhans AD, Schmitz H, Doerr HW.Identification of a novel coronavims in patients with severe acute respiratory syndrome. N Engl J Med, 2003,348:1967 - 1976.
  • 7Marra MA, Jones SJ, Astell CR, Holt RA, Brooks - Wilson A, Butterfield YS, Khattra J, Asano JK, Barber SA, Chan SY, Cloutier A,Coughlin SM, Freeman D, Girn N, Griffith OL, Leach SR, Mayo M, McDonald H, Montgomery SB, Pandoh PK, Petreseu AS, Robertson AG, Schein JE, Siddiqui A, Smailus DE, Stott JM, Yang GS, Plummer F, Andonov A, Artsob H, Bastien N, Bernard K,Booth TF, Bowness D, Drebot M, Fernando L, Flick R, Garbutt M,Gray M, Grolla A, Jones S, Feldmann H, Meyers A, Kabani A, Li Y, Normand S, Stroher U, Tipples GA, Tyler S, Vogrig R, Ward D, Watson B, Brunham RC, Krajden M, Petrie M, Skowronski DM, Upton C, Roper RL. The genome sequence of the SARS-Associated Coronavirus, Sciencexpress , published on line, 1085952,2003.
  • 8Rota PA, Oberste MS, Monroe SS, Nix WA, Campagnoli R,Icenogle JP, Penarunda S, Benkamp B, Mailer K, Chen MH, Tong S,Tamin A, Lowe L, Frace M, DeRisi JL, Chen Q, Wang D, Erdman DD, Peret TC, Burns C, Ksiazek TG, Rollin PE, Sanchez A, Liftick S, Holloway B, Limor J, McCaustland K, Olsen-Rassmussen M, Fouchier R, Gunther S, Osterhaus AD, Drosten C, Pallansch MA, Anderson IJ, Bellini WJ. Characterization of a novel coronavirus associated with severe acute respiratory syndrome. Sciencexpress,published on line, 1085953,2003.
  • 9Saif IJ and Wesley RD, Transmissible gastroenteritis. In: Leman AD, Straw BE, Mengeling WL, D' Allaire S, Taylor DJ (eds.).Diseases of swine, pp.362 - 386, Wolfe Publishing Lid, 1992.
  • 10Enjuanes L, Smerdou C, Castilla J. Development of protection against coronavirus induced diseases. A review. Adv Exp Med Biol,.1995 ,380: 197 - 211.

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第一军医大学学报
2003年 第10期

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