受体介导内吞和自噬同凋亡的关系

Association of receptor-mediated endocytosis and autophagy with apoptosis

目的观察刀豆素A(ConA)与小鼠腹腔巨噬细胞表面ConA受体结合、内吞、转运及巨噬细胞自噬、凋亡的形态学变化,以探讨受体介导内吞与自噬体形成和细胞凋亡之间的关系。方法用辣根过氧化物酶(HRP)标记ConA(ConA-HRP)与小鼠腹腔巨噬细胞共孵育,不同时间取出部分巨噬细胞,制备电镜标本,观察。结果透射电镜观察表明:ConA的内吞属于受体介导内吞;形成的内吞体有泡状、管状及双层膜的线状等形式;双层膜的线状结构包裹部分胞质和细胞器,形成自噬体;自噬体与溶酶体融合,而后巨噬细胞凋亡。结论受体介导内吞和自噬同凋亡之间存在一定的关系。

Objective To evaluate the relationship between receptor-mediated endocytosis, autophagosome formation and apoptosis by observing the morphological changes of mouse peritoneal macrophages in the course of receptor-mediated endocytotic activities, autophagy and apoptosis. Methods Mouse peritoneal macrophages were incubated with horseradish peroxidase-labeled concanavalin A (ConA-HRP), and morphological examinations were performed at different time points after the incubation. Results After the incubation of the macrophages with ConA-HRP, ConA-HRP was observed to enter the vesicles by way of receptor-mediated endocytosis. Three kinds of endosomes were observed, namely vesicles, tubes and double-membrane sheets. The double-membrane sheets enveloped a portion of the cytoplasm and organelles, thus giving rise to vesicles, or the autophagosomes, which later fused with lysosome, followed by the apoptosis of the macrophages. Conclusion Receptor-mediated endocytosis of ConA-HRP is associated with autophagy and apoptosis.