趋化因子在日本血吸虫感染模型中的表达特征

PATTERN OF CHEMOKINE EXPRESSION IN MODEL OF SCHISTOSOMA JAPONICUM INFECTION

目的 研究日本血吸虫感染模型中趋化因子(chemokine)在CD_4^+T细胞中的表达概貌,并探讨其与Th细胞极化和免疫病理的相关性。方法 采用高密度寡核苷酸芯片(Affymetrix芯片)技术,从基因水平获得日本血吸虫不同感染时期CD_4^+T细胞表达的趋化因子概貌,并用反转录PCR(RT-PCR)凝胶成像半定量技术验证嗜酸性粒细胞趋化因子(ECF-L)的表达水平。同时观察趋化因子表达与病理的的关联。结果 日本血吸虫虫卵沉积前,MIG、RANTES明显增强;感染6周IP-10、MIP-2、MIP-1α、MIP-1β、ECF-L升高,持续到感染13周;GRO、Lymphotactin、MCP-1、RANTES在感染13周显著增强。RT-PCR半定量验证ECF-L表达模式与基因芯片结论一致。结论 日本血吸虫人工感染小鼠的CD_4^+T细胞表达大量趋化因子,它们共同参与Th1/Th2免疫应答,影响免疫病理状态;改变趋化因子格局可能影响疾病的自然转归。

Objective To study the changes of chemokine expression on a genomic scale in CD_4^+ T cells of mice infected with Schtstosoma japonicum and their roles in type 1 and type 2 responses and the pathogenesis. Methods The cDNA microarray technique (Affymetrix) was used to explore the chemokine gene expression profile of CD_4^+ T cells from normal and infected mice with Schistoso-ma japonicum in different periods. RT-PCR was used to verify the expression level of eosinophil chemotactic factor (ECF-L) which had been detected in Genechip. Results In the CD_4^+ T cell of infected mice, MIG and RANTES remarkably increased at 3 weeks. IP-10,MIP-2,MlP-lα,MIP-lβ.ECF-L were upregulated in 6 weeks and maintained to 13 weeks.GRO,lymphotactin,MCP-1,RANTES were reinforced in 13 weeks.The result of RT-PCR demonstrated the same with the Genechip.Conclusions There are patterns of coordinate chemokine expression characteristic in CD_4^+ T cells during the infection of Schistosoma japonicum, that may influence the regulation between type 1 and type 2 responses. Expression profile of chemokines may determine the character of an inflammatory response independently of the dominant pattern of differentiation of antigen-specific T cells.