连续切片加免疫组化染色法在乳腺癌微灶转移检测中的应用

Application of sequential pathological section and immunohistochemical staining in detection of micrometastasis of breast carcinoma

目的:推荐一种检测恶性肿瘤微灶转移的方法。方法:应用连续切片的免疫组化法回顾研究50例乳腺癌的淋巴结微灶转移情况,将结果与常规病理切片法进行配对资料的卡方检验,并统计微灶转移率。结果:常规病理切片法的转移灶检出率为30%,连续切片的抗细胞角蛋白免疫组化法的转移灶检出率为50%,二者间差异有显著性。连续切片法发现的微灶转移率为28.5%。结论:对于肿瘤的微灶转移的发现,连续切片的免疫组化法更有优势,它是一种敏感的检测微灶转移的方法。

Objective: To evaluate an assay that can detect the micrometastasis in the carcinomatous patients. Methods: Sequential pathologic section and innnunohistnchemical staining were applied on 50 breast carcinoma cases to study the occult micrometastasis by re-examined. The paired data x2 test was performed, and calculated the micrometastatic rate of tumor.Results: Occult micrometastatic rate is 30% in conventional pathologic section, and is 50% in sequential pathologic section with anti - cytokeratin immunohistochemical staining. There is a significant difference between both groups. The micrometastatic rate in all groups is 28.5 % . Conclusion: Detection of occult metastasis of tumor, sequential pathologic section is superior to conventional section, suggesting sequential pathologic section is a more sensitive method to detect the mi-crometastatasis of tumor.