南京血吸虫感染兔尿液中的虫卵观察

OBSERVATIONS ON SCHISTOSOME EGGS IN BLADDER URINE OF A SCHISTOSOMA NANJINGI-INFECTED RABBIT

目的 观察和计数南京血吸虫(Schistosoma nanjingi)感染兔尿液中的虫卵和孵出的毛蚴。方法 兔感染S.nanjingi尾蚴1 000条,75 d后解剖,取出膀胱,观察和取出膀胱表面静脉丛中血吸虫,观察和计数膀胱内尿液中的血吸虫卵,尿液水洗后孵化及计数毛蚴。结果 膀胱表面静脉丛中取出血吸虫14对(28条),膀胱中尿液发现虫卵18 840只,卵壳表面有很小的颗粒状附着物,兔尿中有变性卵和变黑卵。尿液水洗后孵化,共孵出毛蚴874只,占虫卵总数的4.6%。结论 S.nanjingi与S.japonicum不同,兔感染S.nanjingi后,尿液中有很多血吸虫卵,且能孵出毛蚴。

Objective To observe and count the number of schistosome eggs and miracidia hatched from bladder urine of a Schistosoma nanjingi-infected rabbit. Methods After the rabbit was infected with 1 000 5. nanjingi cercariae and dissected on day 75, the bladder was removed and the arlult worms in venous plexus of the bladder were counted, and schistosome eggs in the bladder urine were observed and counted. After washing the eggs with water, the eggs were incubated at 26 C , and then miracidia hatched were counted. Results The number of adult worms in bladder venous plexus was 14 pairs (28 worms) and 18 840 eggs were found in the bladder urine. The surface of eggs adhered to a lot of small granular substance. Some degenerated and black eggs were seen in the urine. The number of miracidia hatched was 874, covering 4. 6% of the total number of the eggs. Conclusion S. nanjingi is different from 5. japonicum. After the rabbit was infected with S. nanjingi a lot of schistosome eggs were shown in the urine and miracidia can be hatched out.