环磷酰胺作用下釉原蛋白在大鼠牙髓及牙髓内新生硬组织中的表达

Expression of Amelogenin in Rat Dental Pulp and New Hard Tissue during the Using of Cyclophosphamide.

目的 :探讨釉原蛋白对牙髓组织的生物学作用。方法 :给大鼠一次性投与环磷酰胺 ,阻碍切牙牙本质的形成 ,对在投药后牙髓组织发生的各种变化与釉原蛋白的关系 ,进行组织学和免疫组织化学的研究。结果 :在切牙唇侧牙髓的形成端见到广泛的细胞水肿样变 ,成牙本质细胞变性、坏死、消失 ;在牙髓侧随着时间的延长形成了骨样牙本质 ;在新生硬组织的周围及其基质中 ,观察到釉原蛋白免疫反应阳性物质的表达。结论 :结果表明釉原蛋白具有诱导牙髓组织形成硬组织的作用 ,这对釉原蛋白硬组织诱导能研究提供了实验依据。

Objectives:To discuss the relationship between the amel og enin diffused to pulp side and dentin generation using cyclophosphamide.Methods:3-week-old rat was selected and injected in cyclophosphami de all in one time for inhibiting the development of dentin in incisor,and then studied the relationship between the varies of pulp tissue generation and amelog enin by histology and immunohistochemistry.Results:Control group:Mature Enameloblast exhibited special percial periodic change:SA legion a nd RA legion presented alternately .Experiment group: lots of cells because swel ling in the pulp side of incisor in rat.Odontoblasts were present with degenerat ion necrosis or disappearance.Enameloblast layer was lined directly with pulp tissue due to odontoblast disappearence.Some osteoid dentin was present near the pulp side.The positive material of amelogenin immunological reaction was observ ed around new tissue and in matrix,some new hard tissue formed in labial side of pulp.Conclusions:Amelogenin has effect of inducing pulp t issue to form hard tissue.It could provide experiment evident for studing induct ion ability of amelogenin.