针刺对肥胖大鼠瘦素、胰岛素和神经肽Y含量的影响

Effect of acupuncture on contents of leptin,insulin and neuropeptide yin obese rats

目的 :观察针刺对肥胖机体瘦素 (leptin)、胰岛素INS和神经肽Y(NPY)含量及其血脑转运的作用。方法 :分别将针刺大鼠放入固定器中 ,取一侧“后三里”和“内庭”穴针刺 ,并接通电针仪。每次10min每日 1次左右轮换、连续治疗 14天。结果 :肥胖大鼠体重、Lee’s指数、体脂 ,血中瘦素、INS和NPY以及下丘脑NPY的含量均显著高于正常大鼠 ,而下丘脑瘦素和INS含量却低于正常大鼠。针刺治疗取得良好减肥疗效的同时 ,肥胖大鼠血中瘦素、INS和NPY以及下丘脑NPY的含量均显著回降 ,而下丘脑瘦素和INS含量却显著回升。结论 :肥胖机体瘦素、INS和NPY血脑转运异常可能是产生肥胖的重要原因。针刺对肥胖机体中枢和外周瘦素、INS和NPY水平的良性调整作用可能是针刺减肥作用机制之一。

Objective:It is to explore the effect of acupuncture on the contents and blood-brain transport of leptin,insulin(INS)and neuropeptide Y(NPY)in obese organism.Methods:The changes of body weight,lee's index,fat,levels of central and peripheral leptin,INS and NPY in obese rats were observed before and after acupuncture.Results:The body weight,lee's index,body fat,the contents of blood leptin,INS and NPY as well as hypothalamic NPY in the obese rats were all markedly higher than those in the normal rats,but the contents of hypothalamic Leptin and INS in obese rats were all lower than of normal rats.After acupuncture treatment the marked weight lose effect was achieved while the contents of blood leptin,INS and NPY as well as hypothalamic NPY obviously decreased,the contents of hypothalamic leptin and INS obviously increased in obese rats.Conclusion:The abnormal tramsport of blood-brain of leptin,INS and NPY be an important reason for the obesity.The good regulation of acupuncture on levels of central and peripheral leptin,INS and NPY possibly were one of mechanism in the antiobesity action of acupuncture. Author's address:Nanjing University of Traditional Chinese Medicine,Nanjing 210029,China