虾夷马粪海胆体腔细胞的类型及功能

Type and function of coelomocyte in sea urchin Strongylocentrotus intermedius

取平均壳径分别为1 9cm和4 4cm虾夷马粪海胆(Strongylocentrotusintermedius)进行实验观察。结果表明,海胆体腔有2种类型的细胞,即变形吞噬细胞和色素细胞。变形吞噬细胞形状不定,能伸出伪足,核较大,线粒体、溶酶体等细胞器丰富。色素细胞具突起,内有紫红色颗粒,颗粒溶于酒精等多种溶剂中,使得电镜下细胞内含有大量空泡,细胞核很少见,细胞器较少。变形细胞离体后可凝集,具吞噬酵母的能力,吞噬能力与温度成正相关。色素细胞具有辅助的免疫功能。

The Strongylocentrotus intermedius individuals were divided into two groups for two different trials, for which the average diameters were 1.9 cm and 4.4 cm, respectively. The results show that there are two kinds of cells in the coelom,which are amoeboid phagocytes and pigment cells. The amoeboid phagocytes are unshaped, which contains big nucleus, many mitochondria and lysosome. The nucleus and cell organelle can′t be observed in the pigment cells under the electron microscopy because there are many purple pigment granulars which can dissolve in many kinds of solutions like sea water and alcohol, that leads to the appearance of empty bubbles in the pigment cells. The amoeboid phagocytes have the abilities to agglutinate in vitro and swallow yeast. The relationship between the swallowing ability and temperature is consistent. The pigment cells have the character of supplementary immune function.