高效液相色谱法测定手参肾宝胶囊中腺苷的含量

Quantitative Determination of Adenosine in Shoushen Shenbao Capsule by HPLC

目的 测定手参肾宝胶囊中腺苷的含量。方法 采用高效液相色谱法。色谱柱为KromasilC1 8柱 (2 5 0mm× 4 .6mm ,5 μm) ;磷酸盐缓冲液 (pH6 .8取 0 .2mol·L-1 磷酸二氢钾溶液 2 5 0ml,加 0 .2mol·L-1 氢氧化钠溶液 1 1 8ml,用水稀释至 1 0 0 0ml,即得 ) -四氢呋喃 (99∶1 )为流动相 ,流速 :1 .0ml/min ;检测波长 2 6 0nm。结果 腺苷在 0 .0 4～ 0 .4 μg范围内具有良好线性关系 ,平均回收率为 97.6 1 % ,RSD =1 .2 8%。

Aim To determine the content of adenosine in Shoushen Shenbao Capsule.Methods The HPLC analysis was carried out on Kromasil C 18 column (250mm×4.6mm,5μm),the mixture of the phosphatic buffer solution(pH6.8,mix 118ml of 0.2mol·L -1 solution of sodium hydroxide with 250ml of 0.2mol·L -1 solution of potassium dihydrogen phosphate,then dilute to 1 000ml with water-(99∶1) was used as the mobile phase.The flow rate was 1.0ml/min.The detection wavelength was 260nm.Results The linear range of Adenosine was 0.04～0.4μg (r=0.999 8),the average recovery of this method was 97.61% and the RSD was 1.28%.Conclusion This method can be applied in quantitative determination of the preparation.