大鼠局灶缺血预处理诱导的脑缺血耐受中热休克蛋白70的表达及其意义

Expression of heat shock protein 70 and its significance in cerebral ischemic tolerance induced by focal ischemic preconditioning in rats

目的 研究局灶脑缺血预处理对热休克蛋白 70 (HSP70 )表达和脑缺血耐受的影响。方法 SD大鼠随机分为 3组 :预缺血组、假手术组及对照组 ,前两组分别在 2小时大脑中动脉缺血 (MCAO)前 3天给予10分钟的预缺血或假手术 ,MCAO后 2 4小时处死 ,对照组给予两次相隔 3天的假手术 ,比较各组梗死体积及HSP70的表达。结果 预缺血组梗死体积较假手术组减少 5 2 5 4 % (P <0 0 1) ,HSP70表达高于假手术组及对照组 (P <0 0 1)。结论 10分钟大脑中动脉预缺血可有效诱导缺血耐受 ,增加HSP70表达。

Objective To investigate the influence of focal ischemic preconditioning on the expression of heat shock protein 70(HSP70) and ischemic tolerance Methods 42 SD rats were divided into 3 groups in which control group received sham operation only, and the other two groups received 2 hours of middle cerebral artery occlusion(MCAO) followed by 22 hours of reperfusion with or without 10 minutes of ischemic preconditioning 3 days before Infarct volume and HSP70 immunoreactivity were evaluated in each group Results Compared with the sham operated group, there was a smaller infarct volume( P <0 01) and stronger HSP70 immunoreactivity( P <0 01) in preconditioning group Conclusion 10 minutes of MCAO can induce ischmic tolerance and increase HSP70 expression Up regulation of HSP70 may play an important role in the molecular mechanism of focal cerebral ischemic tolerance