摘要
利用作者已测定的赤麂 (Muntiacusmuntjak)线粒体全基因组序列和从GenBank检索到的鲸偶蹄类有代表性的长须鲸、河马、奶牛、绵羊、猪、羊驼 6种动物的线粒体全基因组序列 ,按顺序分别连接各自的 13个蛋白编码基因、 13个氨基酸序列、 2个rRNA基因和 2 2个tRNA基因成一个氨基酸序列或核苷酸序列 ,用DNASTAR软件统计碱基长度和组成 ;分析奶牛、绵羊和赤麂两两之间蛋白编码基因的序列差异 ;用MEGA计算 7种动物 12S和 16SrRNA基因的遗传距离 ;基于连接在一起的 13个蛋白编码基因的氨基酸序列 ,用NJ法构建系统关系树。结果显示 :①偶蹄目反刍亚目牛科的绵羊和同亚目鹿科的赤麂先聚为一亚支 ,然后与该亚目牛科的奶牛并为一支 ;猪形亚目猪科的猪和胼足亚目驼科的羊驼并为一支 ;鲸目须鲸亚目须鲸科的长须鲸和偶蹄目猪形亚目河马科的河马并为一支。②赤麂与绵羊的亲缘关系更近。③推测赤麂与绵羊和奶牛的分歧时间分别约在 14 7和 16 0百万年前。
Using the mitochondrial genomes of the muntjak (Cervidae:Muntiacus) sequenced by our lab.and 6 other species of Cetartiodactyla obtained from the GenBank,we calculated the sequence lengths and base compositions of their individual concatenated sequences of 13 amino acid sequences,13 protein-coding genes,2 rRNA genes,22 tRNA genes and control region.Sequence differences of protein-coding genes between cow/sheep,sheep/muntjak and muntjak/cow were analysed and genetic distances of the concatenated 12S rRNA and 16S rRNA genes among the 7 Cetartiodactyla species were calculated with MEGA.Based on the contatenated sequences of 13 inferred amino acid sequences of protein-coding genes,we constructed the phylogenetic tree with Neighbor-joining method,and discussed their phylogenetic relationships.Our results show:①those species are divided into 3 clades which are a muntjak-sheep-cow clade,a pig-alpaca clade and a hippopotamus-whale clade;sheep and muntjak are first grouped into one branch and then cow is added to them;②there is a closer relationship between muntjak and sheep than that betweeen muntjak and cow;③the divergence between the muntjak and sheep lineages occurred at 14.7 million years ago and the one between the muntjak and cow lineages did at 16.0 million years ago.
基金
国家自然科学基金资助项目 ( 3 9970 11)
