稀有濒危植物翅果油树种群黄酮类化合物多样性研究

STUDY ON THE DIVERSITY OF FLAVONOIDS IN RARE-ENDANGERED ELAEAGNUS MOLLIS POPULATION

以稀有濒危植物翅果油树叶中黄酮类化合物为标记 ,探索翅果油树种群与其生境的相互关系。通过对濒危植物翅果油树的主要分布区 ,山西省的乡宁、翼城、平陆等三种群及环境的调查 ,并采集三个种群的 2 4个样品。用水提法、甲醇提法 ,经聚酰胺薄层层析法分离 ,并将层析结果作 1 /0的二元数据化处理 ,用于聚类分析和PCA排序。结果表明 :1 )翅果油树叶中黄酮类化合物的种类比较稳定 ,可以作为研究种群分化的标记物 ;2 )聚类分析表明乡宁、翼城、平陆三个种群间所含的黄酮类化合物已有分化 ,乡宁种群与翼城种群之间的相异性较小 ,在截取值为2 0时 ,乡宁与翼城种群首先聚为一支 ,当截取值为 2 5时 ,平陆种群归并 ,表明平陆种群与其他种群的相异性较大 ;3)各种群内个体间的共享率有明显的差异 4)PCA分析表明 :不同种群内的黄酮类化合物的差异与其地理位置、温度、地形地貌及土壤养分等因子有关。

The flavonoids is used molecular marker to study the relation between natural population of Elaeagnus mollis and the habitat. The flavonoid , which is important secondary metabolit that is ubiquitous in plant, was extracted by the methynol and water from the leaves of Elaeagnus mollisand was isolated by polyamide thin-layer chromatography. The band spectrum was statistical and analyzed by hierarchical cluster and PCA analysis. The reasult showed 1)that in E. molls population was stable and was as marker for studying population differentiation of E. molls. 2) Cluster analysis indicated That the flavonoids differentiation was into among 3 populations. The dissimilarity between Xiangning and Yicheng population was small by clustering in 20 value but Pinglu population in 25 value. 3) The share ratio is obviously different among populations. 4) PCA analysis indicated that flavonoids difference among population is the relationship to its environmentail factors.