胚胎大鼠背根神经节细胞的分离培养、纯化及生物学特性的研究

PURIFICATION AND CHARACTER OF CULTURED EMBRYONIC DORSAL ROOT GANGLION CELLS

本实验取E15 SD胎鼠的背根神经节,用胰蛋白酶消化分离成单细胞,在NB1培养基中培养,并通过差速贴璧法进行背根神经节神经元(DRGn)的分离纯化,用神经元特异性的烯醇化酶(NSE)鉴定培养的神经元。结果发现DRGn在体外合适条件下可存活3-4周,DRGn纯化培养的纯度达91％左右。DRGn在体外能存活较长时间,可作为神经科学研究的细胞模型。

Dorsal root ganglions from E15 Sprague Dawley embryonic rats were digested with trypsin and the cell suspension was cultured in NB1 media. Dorsal root ganglion neuron (DRGn) cells were purified by differential adhesion and identified using neuronal specific enolase (NSE) immunocytochemistry stain. DRGn cultured under suitable conditions maitained 3-4 weeks in vitro. The purification rate of purified DRGn by differential adhesion arrived at 91 % . DRGn cultured in vitro can survive longer time and act as a useful cell model in Neuronoscience research.