绞股蓝丹对大鼠实验性体内血栓形成的抑制作用研究

Study the Effect of Gynostemma Pentaphyllum Makin in Restraining Thrombosis in Vivo of Experimental Mice

目的 :观察绞股蓝丹对大鼠实验性体内血栓形成的抑制作用。方法 :大鼠灌服绞股蓝丹低、中、高 3种剂量(2 0 g/kg、4 0 g/kg、6 0 g/kg)后 ,采用大鼠实验性动静脉旁路血栓形成和电刺激颈总动脉形成血栓两种方法 ,观察绞股蓝丹对血栓形成的影响。结果 :用动静脉旁路血栓形成造模后 ,血栓重量分别为 (2 5 .6± 3.9) m g、(2 2 .4± 3.3) m g、(2 2 .0± 3.6 ) m g,与对照组 (2 9.0± 3.1) mg比较有非常显著差异 (P<0 .0 1) ,血栓形成抑制率分别为 11.7%、2 2 .8%和 2 4 .1% ;治疗组电刺激大鼠颈总动脉后血栓形成的时间 (OT)值分别为 (112 8.5± 346 .5 ) s、(876 .2± 133.2 ) s、(778.5± 76 .3) s,与对照组 (6 96 .6± 4 4 .5 ) s比较有非常显著差异 (P<0 .0 1)。结论 :绞股蓝丹具有抗大鼠体内血栓形成的作用。

Objective:To observe the effect of gynostemma pentaphyllum makin in restraining thrombosis in vivo of experimental mice.Methods:Gynostemma pentaphyllum makin had been affused in the stomach of experimental mice in three dosages:20g/kg,40g/kg,60g/kg.Apply the way of by-passed artery and vein and the way of electricity stimulation in experimental mice to forme thrombosis,observe the effect of gynostemma pentaphyllum makin in the thrombosis.Results:The weight of thrombosis was 25.6±3.9mg,22.4±3.3mg and 22.0±3.6mg repectively in the models of by-passed artery and vein,and had significant difference in comparison with the controlled group (29.0±3.1mg)(P<0.01),the rate of restraining thrombosis was 11.7%,22.8% and 24.1%;The time of thrombosis (OT) was 1128.5±346.5s、876.2±133.2s、778.5±76.3s in comparison with the controlled group (696.6±44.5s) and there was a significant difference between them (P<0.01).Conclusion:The drug,gynostemma pentaphyllum makin,can be used in restraining the thrombosis of experimental mice. [