α-干扰素对慢性粒细胞白血病来源的树突状细胞的免疫调节作用(Ⅰ)

Immunoregulatory effects of interferon-α on dendritic cells from patients with chronic myeloid leukemia (I)

目的 于慢性粒细胞白血病 (CML)来源的树突状细胞 (DCs)无血清培养基中 ,加入α 干扰素 (IFN α) ,以探讨IFN α对CML DCs表达共刺激分子及分泌IL 10、IL 12P70的影响。方法 在诱导CML DCs的培养基中 ,除加入SCF、GM CSF、TNF α及IL 4外 ,还加入不同浓度IFN α。培养 10～ 14d后 ,流式细胞仪检测细胞免疫表型 ;G显带法显示Ph1染色体 ;噻唑蓝 (MTT)法检测CML DCs刺激正常人外周血淋巴细胞增殖状况 ;ELISA法检测培养上清IL 10及IL 12P70含量。结果 IFN α(30 0U·mL-1)组较无IFN α组 ,CML DCs的CD4 0、CD83、CD86及MHC I类抗原的表达均升高一倍 ;异体混合淋巴细胞反应 (MLR)中OD值增加一倍 ;Ph1染色体阳性比例、IL 10及IL 12P70浓度均减低。结论 IFN α能够部分纠正CML DCs免疫表型及功能缺陷 ,同其解除了CML血清中IL 10对CML

Objective To explore the effects of interferon-α(IFN-α) on expressions of costimulatory molecules and secretions of interleukin-10 (IL-10) and IL-12 P70 in dendritic cells (DCs) of chronic myeloid leukemia (CML). Methods In addition to adding stem cell factor (SCF), granulocyte-macrophage-colony-stimulating factor (GM-CSF), tumor necrosis factor-α(TNF-α) and IL-4, IFN-α was added to the serum-free medium of DCs. After 10～14ds' culture, phenotypes and function of CML-DCs were evaluated, respectively, by flow cytometry and methyl thiazolyl tetrazolium (MTT) assay. Chromosome analysis of DCs was made by displaying G banding assays. The concentrations of IL-10 and IL-12P70 in supernatants were evaluated by enzyme-linked immunosorbent assay (ELISA). Results Expressions of CD40, CD83, CD86 and major histocompatibility complex (MHC)-I antigen molecules and the OD number in allogeneic mixed-lymphocyte reaction (MLR) in IFN-α(300U·mL -1) group were twice as high as those in non- IFN-α group. The percentage of Ph 1positive cells and concentrations of IL-10 and IL-12 P70 were reduced in IFN-α group. Conclusion Defective phenotypes and functions of CML-DCs can be corrected partly by IFN-α. The mechanism may lie in the fact that the inhibitory effect of IL-10 on CML-DCs is relieved partly through the regulation of IFN-α.